Possible Interaction: Ethanol and Aminopyrine

“The aminopyrine plasma disappearance rate, determined by GLC, decreased by 30% after ethanol (p less than 0.05).”

“ Ethanol increased the microsomal lauric acid omega-hydroxylation and the aminopyrine N-demethylation catalyzed by cytochrome P450.”

“The addition of antipyrine or aminopyrine to isolated hepatocytes derived from normal rats and incubated with ethanol caused a significant decrease in the oxidation of ethanol to acetate.”

“The EtOH alone tended to increase the microsomal parameters such as cytochrome P-450 content and enzyme activities of aniline hydroxylase, aminopyrine demethylase and UDP-glucuronyl transferase, while it did not affect the cytosolic enzyme activities of sulfotransferase and glutathione S-transferase.”

“In contrast, this dose range of ethanol inhibited aminopyrine accumulation stimulated by forskolin or dibutyryl-cyclic adenosine monophosphate.”

“ Alcohol feeding also induced aminopyrine N-demethylase activity measured in vitro.”

“ Ethanol also inhibited aminopyrine demethylation in liver homogenates from ADH- and ADH+ animals in a dose-dependent manner and to a comparable degree in both strains.”

“ Ethanol feeding caused 4- to 5-fold increases in rates of the microsomal metabolism of aniline and p-nitrophenol, and smaller increases in rates of dealkylation of 7-ethoxycoumarin and aminopyrine.”

“Exposure of cultured chick embryo hepatocytes to ethanol , isobutanol, or isopentanol, the predominant alcohols present in commercial alcoholic beverages, resulted in increased metabolism of aminopyrine or biphenyl by the intact cells.”

“In isolated hepatocytes from fasted rats ethanol inhibited CO2 production from aminopyrine , but not from formaldehyde or formate.”

“The production of [14C]O2 from 14C-labeled aminopyrine involves the following sequence: Aminopyrine 1 leads to formaldehyde 2 leads to formate 3 leads to CO2 Ethanol has the potential to affect any of the above steps in this sequence.”

“N-Demethylation of aminopyrine (P-450 mediated) was increased both in vivo and in vitro in rats after chronic ethanol feeding (P less than 0.05) whereas in vivo N-demethylation of caffeine and O-dealkylation of phenacetin (P-448 mediated) were unchanged in the same animals.”