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“Ruthenium red at a concentration that decreased calcium uptake by greater than 90 per cent did not affect calcium outflow; whereas ethacrynic acid (5 × 10 −4 M) induced a significant increase in calcium loss.”
“ CA is at least 10 3 times more active than N -ethyl-maleimide (a SH-reagent of similar reactive structure) and ethacrynic acid (a hydrophobic SG-reagent which readily inactivates most membranous enzymes) [14].”