Possible Interaction: Epoprostenol and Arachidonate

“Inclusion of arachidonate in the diet augmented both prostacyclin and thromboxane production by 25 to 35%.”

“ Prostacyclin production of human adult endothelial cell cultures was stimulated by aracidonate and thrombin by 17 to 20 and 2 to 3-fold respectively.”

“ PGI2 production under successive treatments with AA or ionophore was reduced by only approximately 30% after each treatment.”

“Infusion of PGI2 at a dose of 2 mg/kg/min during 72 hours into the patients caused the decreased platelet aggregability to ADP and arachidonate but not to collagen, and a decreased tendency to production of TXA2 in PRP aggregated by arachidonate.”

“When the medium was supplemented with 30 microM arachidonate (saturating concentration), prostacyclin release still significantly decreased after 30 min of hypoxia but returned to baseline levels by 30 min of reoxygenation.”

“A23187 and arachidonate increased both endothelial and smooth muscle prostacyclin production (P < 0.01).”

“Addition of arachidonate to food enhanced TXA2 and PGF2 alpha releases and decreased PGI2 and PGE2 releases, while inhibiting the food-induced increases in JBF and O2 uptake; mefenamate inhibited these arachidonate actions.”

“Formation of PGI2 , as well as TXA2, as measured by radioimmunoassay (RIA) of their metabolites, was increased in atherosclerotic aortic segments relative to nonatherosclerotic segments (P less than or equal to 0.05) at 0, 5, 10, 15, and 30 min of incubation with arachidonate.”

“The valve subendothelial cells also released 10-fold more prostacyclin in response to arachidonate than did skin fibroblasts or smooth muscle cells.”

“The aggregation of the platelets to arachidonate was inhibited by cyclooxygenase inhibitors and by prostacyclin.”