Possible Interaction: Eicosapentaenoic Acid and Hydrogen Peroxide

“ EPA and DHA significantly reduced intracellular reactive oxygen species under both basal condition and H2O2 stimulation.”

“Furthermore, treatment with EPA reduced hydrogen peroxide (H2O2)-induced cell death.”

“In addition, EPA restored the phosphorylation of Akt activated by H2O2 treatment and induced the phosphorylation of AMPK in H9C2 cells under oxidative stress.”

“The cells could be killed in a synergistic manner following exposure to combinations among H2O2 and the following membrane-damaging agents: streptolysins S (SLS) and O (SLO), poly-d-lysine, arachidonic acid, eicosapentanoic acid , arachidic acid, lysophosphatidylcholine, lyso-phosphatidylinositol, lysophosphatidylglycerol, ethanol, and sodium taurocholate.”

“Interestingly, hydrogen peroxide was able to mimic the effect of EPA and DHA in decreasing the expression of PGHS-1.”

“In addition, both ALA and EPA enhanced the formation of superoxide anion, hydrogen peroxide and lipid peroxides, and caused a reduction in the levels of antioxidant enzymes: SOD, catalase and glutathione peroxidase and induced significant damage to DNA in SP 2/0 cells.”

“The present study was undertaken to examine whether the oxidative effect of organic hydroperoxide on cultured rat hepatocytes is enhanced by eicosapentaenoic acid (EPA).”

“Moreover, through the biosynthesis route employing l-amino acid oxidase, the byproduct hydrogen peroxide leads the degradation of IPA.”

“Treatment of the cells with 0.1 mM H2O2 resulted in much lower intracellular concentrations of H2O2 being consistently detected in cells with EPA than in those without EPA.”

“We discuss the possible role of EPA in partially blocking the penetration of H2O2 into cells through membranes containing EPA.”