Possible Interaction: Eicosapentaenoic Acid and Acetylcholine

“The FBF response to acetylcholine (but not to nitroprusside) was significantly less in HTG subjects before EPA than in control subjects.”

“Therefore, EPA treatment improved the coronary vasomotor responsiveness to ACh , but did not inhibit ACh-induced coronary vasospasm.”

“We examined whether purified eicosapentaenoic acid (EPA), a major component of fish oil, improves the coronary vasomotion in response to acetylcholine , and the effect of purified EPA on acetylcholine (ACh)-induced coronary vasospasm in 22 patients with variant angina.”

“The long-term treatment with EPA significantly improved the FBF responses to acetylcholine (p < 0.01), which was significantly reduced by acute administration of L-NMMA (p < 0.01).”

“The long-… treatment with EPA augmented forearm blood-… response to both acetylcholine and substance P. Furthermore, acute administration of L-NMMA significantly inhibited the EPA-induced augmented response to acetylcholine but not that to substance P. The forearm vascular response to sodium nitroprusside was unchanged by the EPA treatment.”

“It was found that eicosa-5,8,ll,14-tetraynoic acid (30–60 μmole/liter) and eicosa-5,8,11,14,17-pentaynoic acid (4–50 μmole/liter) decrease the amplitude of the inward current induced by application of acetylcholine , and attenuate its extinction during repeated applications.”

“We conclude that dietary supplementation with EPA inhibits endothelium-dependent dilations to ACH in guinea pig coronary microvessels.”

“In the ileal and colonic LSMs, DHA and EPA (3 × 10-5 M each) significantly inhibited contractions induced by acetylcholine (ACh), histamine, and prostaglandin (PG) F2α (vs. control), and these effects are stronger than that of LA (3 × 10-5 M).”