Possible Interaction: Edetic Acid and Vitamin C

“ EDTA prevented the loss of binding normally seen with ascorbic acid at a tissue concentration of 17 micrograms/ml.”

“ Plasma ascorbic acid , as measured using HPLC with electrochemical detection (LC-ECD) decreased by 75% with overnight storage using EDTA as an anticoagulant, but was unchanged when heparin was used.”

“One need only cite the remarkable observation that adding ethylenediaminetetraacetic acid (EDTA) to an enzyme-free system of iron-ascorbic acid markedly speeds up the p-hydroxylation of many aromatic compounds, an effect which one might expect to require the presence of an iron-dependent natural enzyme.”

“When added before AA, EDTA completely inhibited the peroxidative effect of AA or FeSO4, and when added subsequent to AA, EDTA partially prevented the adverse effect of AA.”

“The chelation of zinc ions from the enzyme by EDTA turned ALA-D more susceptible to the inhibitory effect of AA.”

“The presence of EDTA and HCl effectively inhibited degradation of ascorbic acid during the collection of urine specimens.”

“However, ascorbic acid prevents the mutagenicity of MNNG in vitro if added to bacteria in a medium prepared with either sterile tap water or deionized water and Cu2+ ions and that this antimutagenic response is blocked by EDTA.”

“The results are consistent with the hypothesis that CA and AA can exert their effects in the presence of EDTA.”

“These simultaneous increases in the lipid peroxidation and the lysosomal enzyme release were completely inhibited by adding ethylenediamine tetraacetic acid , indicating that the ascorbic acid-induced lipid peroxidation is responsible for the labilization of lysosomes.”

“The simultaneous increases in lipid peroxidation and renin release induced by ascorbic acid in the renin granule fraction were markedly suppressed by the addition of disodium ethylenediaminetetra-acetic acid and antioxidants such as N,N'-diphenyl-p-phenylenediamine and hydroquinone.”