Possible Interaction: Edetic Acid and Trypsin

“The mean number of cells … biopsy was higher after trypsin: EDTA digestion of a dermis‐containing biopsy using a trypsinizing flask (4.0x106 cells/cm2) compared to a biopsy where … been separated by thermolysin (2.8x106 cells/cm2), Dispase (2.3x106 cells/cm2) or trypsin (1.1x106 cells/cm2).”

“Although apoptosis assay kit was applied by many companies, the manufacturers were not consistent with whether using Trypsin with EDTA to collect the adherent cells.”

“In particular, trypsin 5 min treatment with ethylene diamine tetraacetic acid (EDTA) showed about 5 times %DNA in the tail value compared to without EDTA treatment.”

“The combination of trypsin with EDTA provided the highest yield of monodispersed islet cells (963 cells/islet) and highest viability (88%).”

“ Trypsin was inhibited by the chelators EDTA and EGTA, a specific effect reversed by either calcium or magnesium.”

“A chelating agent, EDTA (ethylenediaminetetraacetic acid disodium salt: dihydrate) at 400 mg l(-1) enhanced the activity of trypsin.”

“Cell dissociation was possible when trypsin and EDTA were given simultaneously, when trypsin was given after EDTA treatment, but not when trypsin was given before EDTA treatment.”

“The presence of EDTA in the medium inhibited recovery from treatment with trypsin and beta-galactosidase; return to normal after exposure to phospholipases A, C and D was slightly affected, whereas recovery from treatment with neuraminidase was unaffected.”

“Their activity was sensitive to 6 mM EDTA and to heating for 5 h at 37 degrees C, but resisted treatment with 0.5 percent trypsin.”

“Enzymatic digestion with trypsin , pronase or neuraminidase, heating at 56°C and treatment with EDTA or nucleotides significantly diminished the binding activity.”