Possible Interaction: Edetic Acid and Imipenem

“The isolates grew well in the presence of 0.4 mM EDTA which reduced the MICs of imipenem and meropenem 16and 32-fold to 0.25 and 0.06 mg/L, respectively.”

“ EDTA could reduce 4 folds or more minimum inhibitory concentrations (MICs) of imipenem in 105 isolates (62.9%).”

“An MBL Etest (bioMérieux) revealed a 24-fold reduction of imipenem MIC when combined with EDTA , which suggested production of an MBL.”

“The MiC of imipenem 32 μg/mL and a reduction of the imipenem MiC in the presence of EDTA to >1.0 μg/mL was observed according to the Etest.”

“Enhancement of inhibition zone around imipenem and meropenem discs impregnated with EDTA as compared to those without EDTA confirmed MBL production.”

“ EDTA reduced imipenem hydrolysis at levels similar to those of the blaVIM-1-negative isolates (1.5 to 3 U), suggesting that carbapenemase activities were largely due to VIM-1.”

“… imipenem … EDTA considered a positive result) as well as two in-house techniques: the double-disk synergy test (DDST), using imipenem (10 μg) and EDTA disks (… imipenem (10 μg) disk alone and containing 930 μg EDTA (with a ≥7-mm increase in inhibition zone considered a positive result) (3).”

“The pattern A isolates were mostly of serotype O12 and were highly resistant to imipenem (MICs, 32 to >256 μg/ml), with this resistance decreased eightfold or more in the presence of EDTA.”

“Using the disk diffusion technique it was shown that the membrane permeabilizers ethylenediaminetetraacetic acid , sodium citrate, and sodium polyphosphate increased susceptibility to a range of antibiotics including imipenem , ciprofloxacin, tetracycline, and rifampicin.”

“The isolate was resistant to all antibiotics except amikacin, and displayed an imipenem MIC of 64 mg/L that decreased to 8 mg/L in the presence of EDTA.”