Possible Interaction: Edetic Acid and Deoxycholate

“Addition of 2 mM EDTA to the serum diluent or pretreatment of LPS with 0.5% deoxycholate enhanced the ENC of serum about 25‐fold or 10‐fold respectively.”

“Plasma phospholipase A2 is thermolabile, and the enzyme activity is enhanced by 2 m m sodium deoxycholate and calcium chloride, and inhibited by EDTA.”

“Besides, DOC weakens the inhibiting influence of promethazine and EDTA on both LP systems.”

“The enzyme activity was activated by Ca2+ and sodium deoxycholate , and was inhibited by EDTA.”

“2.By incubation with deoxycholate + EDTA activity of the (Na+ + K+)-activated enzyme increased about 3-fold, while the activity with Mg2+ alone remained unchanged.”

“Since the strains exhibit extreme sensitivity both to deoxycholate and osmotic shock in the presence of EDTA , the increased sensitivity to antibiotics (including mitomycin-C) is probably caused by a change in the cell envelope.”

“A closer examination of this phenomenon revealed that treatment of polysomes by deoxycholate in the presence of EDTA results in the release of part of the structural proteins from the ribosomal subunits.”

“However, in the course of a study of the contamination of polysomes by ribonucleoproteins of nuclear origin [2] , evidence was obtained that when polysomes were treated with deoxycholate in the presence of EDTA , the sedimentation properties of the subunits obtained were lower than expected.”