Possible Interaction: Edetic Acid and Ascorbate

“In some experiments, ascorbate alone induced chromaffin granule lysis which was inhibited by EDTA , EGTA, or deferoxamine.”

“In other experiments, several chelating agents (e.g., EDTA ) or commonly used inhibitors of lipid peroxidation (e.g., propyl gallate) were able to counteract the effects of ascorbate on lipid peroxidation and on [3H]spiroperidol binding.”

“Thus, while each additive, alone, lowered binding of 3H-APO and of 3H-ADTN, ascorbate plus EDTA increased the proportion of specific binding, especially of 3H-APO, and protected both from degradation.”

“In contrast, the slowly developing loss of opioid binding during exposure to 1 mM ascorbate at 23 degrees was completely inhibited by 10(-5)M EDTA or Mn++.”

“Surprisingly, added ascorbate and EDTA accelerated rather than inhibited degradation.”

“On the other hand, Fe2+ EDTA ) greatly augmented both ascorbate autoxidation and ascorbate-dependent .OH production.”

“The metal chelator EDTA showed significant inhibition of ascorbate autoxidation and ascorbate-dependent .OH release.”

“Hydroxyl radicals were generated by autoxidation of ascorbate in the presence of iron and EDTA.”

“In the presence of ascorbate addition of Fe or its complexes with EDTA and resulted in 15-HPAA decomposition, which could be measured by the changes in the UV absorption.”

“It is concluded that ascorbate and glutathione are not effective during extended periods of storage of SFHS, and that EDTA causes formation of large amounts of Hi.”

“DETAPAC, EDTA and HEDTA (N-(2-hydroxyethyl)-ethylenediaminetriacetic acid) are effective at slowing the copper-catalyzed autoxidation of ascorbate while Desferal is ineffective.”