Possible Interaction: Dopamine and Genistein

“The NOS inhibitor N(G)-nitro-l-arginine methyl ester and genistein , wortmannin, or NF-κB SN50, inhibitors of protein tyrosine kinases phosphatidylinositol 3-kinase and NF-κB, respectively, reduced nitrite production by dopamine and SKF R-38393 but were less effective in attenuating H2O2-mediated effects.”

“Furthermore, genistein potently inhibited DA uptake with a K(i) = 68 nM. Kinetic analysis confirmed that genistein decreased the V(max) of the DAT, with no change in K(m).”

“ Genistein increased dopamine levels in the forebrains of B. splendens at both environmentally relevant and pharmacological doses.”

“In rat dorsal striatal synaptosomes, inhibition of tyrosine kinases by genistein or tyrphostin 23 resulted in a rapid (5–15 min), concentration‐dependent decrease in [3H]dopamine uptake because of a reduction in maximal [3H]dopamine uptake velocity and dopamine transporter cell surface expression.”

“ Genistein , a broad-spectrum inhibitor of protein tyrosine kinases, inhibited dopamine uptake into mouse striatal homogenates with an IC50 of 18 microM. The inhibition by genistein was rapid, reversible and somewhat selective, in that genistein did not inhibit the uptake of choline or GABA under similar conditions.”

“Fractional release of DA was significantly increased over basal release levels by genistein (100 and 200 µM).”