Possible Interaction: Dinoprostone and Arachidonate

“A similar stimulatory effect of hyperosmolarity on PGE2 production was also seen when arachidonate was added exogenously.”

“Auranofin (0.2‐2 μΜ) enhanced the response to phorbol ester two‐ to three‐fold, and similar … aurothiomalate, aurothioglu‐ cose, and penicillamine, but only after pretreatment for 1‐4 h. The enhanced mobilization of arachidonate was accompanied by … release of prostaglandin E2 and 6‐keto prostaglandin … of lipoxygenase metabolites.”

“…,PGE2 as well as LTB 4 "de novo" production by corneas and lenses obtained from rabbits sacrificed 2 h after arachidonate and incubation "in vitro" for 20 min were significantly higher in samples taken from controls than in tissues obtained from the eyes treated with sodium naproxen eye drops.”

“Addition of arachidonate to food enhanced TXA2 and PGF2 alpha releases and decreased PGI2 and PGE2 releases, while inhibiting the food-induced increases in JBF and O2 uptake; mefenamate inhibited these arachidonate actions.”

“These results suggest that cyclooxygenase may be involved in this process, indeed added arachidonate , bypassing PLA2, enhanced PGE2 synthesis in PMS-treated cells further supporting the involvement of cyclooxygenase.”

“Since arachidonate increased PGE2 release from the mucosa more than 10-fold and indomethacin did not inhibit this effect, indomethacin at high concentration (0.5-1 mM) appears to also inhibit the action of intestinal secretagogues by a prostaglandin-independent mechanism.”