Possible Interaction: Dimethyl Sulfoxide and Glycerol

“The … GLY … DMSO was shown to be significantly different to CON with 57.1% (95% CI = 21.43–133.34) and 19.1% (95% CI = 11.…–60.27), respectively at week 16, and its use should be encouraged as a cryoprotectant for M. bovis at − 20 and − 80 °C.”

“It was found that dimethylsulfoxide DMSO ) and glycerol drastically reduced intra-membrane particle densities.”

“In addition, the combination of 10% DMSO with 15% glycerol has shown positive effects, whereas other analyzed combinations of additives failed to amplify the EGFR promoter region.”

“The transport system was competitively inhibited by H+, reacting with at least three hydrogen analogue, as well as dimethylsulfoxide (a hydrogen bonding molecule with no structural resemblance to glycerol), inhibited glycerol transport competitively.”

“However, there are important differences between the cryoprotective effects of DMSO and glycerol.”

“The similarities between cryoprotection by DMSO and glycerol reinforce Rapatz and Luyet's classification of cryoprotective agents into three types and support Mazur's two-factor theory of cryoprotection.”

“BACKGROUND There is a possibility to reduce the toxicity of glycerol and osmotic stress of DMSO by lowering their concentrations in freezing extenders.”

“Significant (P < 0.05) differences in total motility and progressive movement between GL and DMSO , EG groups were observed.”

“The 10% GLY + 10% DMSO combination reduced (P < 0.05) frozen-thawed sperm motility, which reached a minimum when 20% GLY + 20% DMSO was used.”

“It may also be noteworthy that Lindinger et al. describe an approximately 50 s delay between solution change and re-initiation of imaging, in which time both DMSO and glycerol addition had produced >10% fibre shrinkage in Fig. 1 here.”

“Sperm motility of 5% DMSO was lower than that of 3% glycerol (P<0.005), and membrane intactness were lower in 5% DMA and DMSO than 3% glycerol (P<0.05).”