Possible Interaction: Dihydroxyphenylalanine and Glutathione

“Abstract: … with l‐DOPA induced a … in GSH level in cultures of fetal rat mesencephalon, mouse neuroblastoma (Neuro‐2A), human neuroblastoma (SK‐N‐MC), pig kidney epithelial cells (LLC‐PK1), and glia from newborn rat brain, but not C6 glioma cells or neuronal cultures (no glia) from the mesencephalon.”

“In addition, l‐DOPA (200 µm) or/and GCM‐treated cells increased their GSH extracellular levels (223, 257, 300% of controls) after 48 h of treatment.”

“When DOPA and glutathione were added to the medium together, the maturation of melanosomes was promoted.”

“Iron or copper ions could also promote conjugate formation between GSH or cysteine and DA and l‐DOPA , especially if H2O2 was present.”

“The decrease in cell viability by L‐DOPA (10±4% of control) or dopamine (15±4% of control) was markedly attenuated by antioxidants (ascorbic acid, glutathione , N‐acetyl‐L‐cysteine and sodium metabisulphite).”

“The relative quantities of GSH at the sites of DOPA quinone formation and the levels of its metabolising enzymes can influence the type of product formed.(ABSTRACT TRUNCATED AT 250 WORDS)”

“It is suggested that GSH may be involved in the steroidogenesis in the inhibition of nonenzymatic decarboxylation of DOPA and in the proteosynthesis in adrenals.”

“The Km values of the three isozymes for L‐DOPA ranged from 8.7 to 10 raM. L‐ascorbic acid and β‐mercaptoethanol, glutathione and sodium diethyldithiocarbamate notably inhibited the enzymatic activities.”