Possible Interaction: Dihydrotestosterone and Vitamin D

“Our findings confirm that exposure to vitamin D based compounds, within LNCaP cells, elicits measurable and significant reduction in the intracrine conversion of DHEA to T, DHT and other intermediate metabolites within the androgenic pathway.”

“Purpose: Vitamin D and dihydrotestosterone pathways interact to promote the growth of prostatic tissue.”

“These data demonstrate that DHT enhances the antiproliferative activity of Vitamin D3 hormones by inhibiting their inactivating enzyme.”

“… 25-hydroxyvitamin D3 at 500 nM significantly increases … increase is significantly decreased by 5alpha-dihydrotestosterone (DHT) at concentrations of 1-100 nM in androgen-sensitive prostate cancer cells LNCaP. 25-Hydroxyvitamin D3 … at 10 nM inhibit LNCaP cell growth, and the growth inhibition is enhanced by 1 nM DHT.”

“After 4 days treatment with vitamin D plus DHT , or pure IGFBP-3, LNCaP cell numbers were increased relative to control.”

“Cell-associated IGFBP-3, and immunoreactive IGFBP-3 species of 20 kDa and 30 kDa were also increased in response to vitamin D plus DHT.”

“… vitamin D … DHT increased DNA synthesis in LNCaP cells 3-fold, and this was at least partly mediated by endogenous IGFBP-3 because anti-IGFBP-3 …, reduced the stimulatory effect of vitamin D and DHT from 293 +/- 11.6% to 161 +/- 30.7% of control levels (P < 0.0001).”

“A pretreatment with dexa, E2, and D3 significantly increased the mitogenic response of HOCs to DHT.”

“We conclude that (1) higher androgen serum levels in males together with a higher AR expression at certain skeletal sites may contribute … development of sex-related differences in skeletal morphology, (2) glucocorticoids induce AR gene expression in HOC cultures, and (3) glucocorticoids, E2, and D3 enhance the … of DHT.”

“When male kidneys preferentially hydroxylate 25-hydroxyvitamin D3 using 24-hydroxylase rather than 25-hydroxyvitamin D3-1-alpha hydroxylase, DHT suppressed the Pgr-mediated 24-hydroxylase expression, and it is important to note that DHT increased the blood 25-hydroxyvitamin D3 levels.”

“Alterations caused by the chronic DHT treatment were prevented partially by concomitant vitD administration.”

“Although the administration of vitamin D3 along with DHT reduced responsiveness to NE, it did not restore relaxation to estradiol.”

“Increased PAR staining in ovaries and circulating leukocytes from DHT rats showed enhanced DNA damage, which was reduced by concomitant vitamin D3 treatment.”

“In the present study, we …,25(OH)2D3 or 24,25(OH)2D3 would …, DHT or progesterone (P) required for stimulation of DNA synthesis and creatine kinase specific activity in cultured osteoblast-… in rat embryo epiphyseal cartilage cells, and to what extent such pretreatment would increase the maximal response.”

“We demonstrated previously that vitamin D metabolites modulate the response of bone and cartilage cells to 17 beta-estradiol (E2) and dihydrotestosterone (DHT) both in cell cultures and in vivo.”