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“The muscle fibres isolated in this way lacked nuclei but they did contract and relax in response to high [K+], a response which was blocked in the presence of Co2+.”
“… tonic fibres the slow action potential had a threshold of ‐50 to ‐60 mV and a peak amplitude of ‐10 mV. In twitch fibres the … of ‐40 mV and reached a peak amplitude of +40 mV. The responses were blocked by the addition of Cd2+ (2 mM) or Co2+ (…).”