Possible Interaction: Diazepam and Calcium Supplement

“Prior administration of DZP (10-6 M) significantly suppressed carbachol (10-7 M)-induced increase in [ Ca2+ ]i. This inhibitory effect was ameliorated by combined use with rebamipide (5 × 10-4 M).”

“ Diazepam (30, 100 µM), but not midazolam (3–30 µM), increased shortening and [Ca2+]i in the presence or absence of L-type Ca2+ channel modulators.”

“ Diazepam (100 microM) enhanced the contractile response of the skinned fiber to calcium and shifted the concentration-response curve to the left.”

“This result suggests that diazepam increases calcium sensitivity of contractile proteins in heart muscles.”

“The Ca2+ -activated and voltage-dependent K+ currents were inhibited by diazepam and midazolam (> 10(-5) M and > 10(-4) M, respectively).”

“ Diazepam effect was significantly reduced in … 3.5 mM calcium medium and KCl vas deferens response was inhibited by diazepam 1.3 x 10(-5) and 1.3 x 10(-4) M. It is concluded that in rat vas deferens diazepam effect seems to be related with calcium mobilization.”

“In synaptosomes depolarized in the presence of added Ca2+ , doses of diazepam ranging from 0.1 to 100 microM were found to significantly enhance Ca2+ levels measured with the fluorescent dye fura-2, compared to control incubations without drug.”

“These results indicate that diazepam is capable of inhibiting calcium , calmodulin-dependent protein kinase activity in astrocytes, thereby suggesting a possible site of diazepam action and a potential mechanism for a role of astrocytes in epileptogenesis.”

“We found that diazepam inhibited calcium , calmodulin-stimulated phosphorylation in both supernatant and membrane fractions from primary cultures of rat astrocytes, whereas phenytoin and valproic acid (50-500 microM) had little to no effect.”

“The change in action potential duration induced by diazepam on mouse preparations was higher than on guinea pig preparations, ca.”

“The decay of the tetanus was prolonged in both types of fibre, which indicated that diazepam blocked calcium uptake by the sarcoplasmic reticulum.”

“ Diazepam does not affect the Ca++ gained during the positive staircase but accelerates the loss of calcium from the residual and the extractable compartments during the initial phase of physiological muscle fatigue.”