Possible Interaction: Dehydroascorbic Acid and Ascorbate

“Intracellular ascorbate accumulated to concentrations of 10-20 mM following loading with DHA.”

“Neither cell type contained appreciable amounts of ascorbate in culture, but both rapidly took up and reduced DHA to ascorbate.”

“However, they readily took up dehydroascorbic acid , thus significantly increasing their intracellular ascorbate concentrations, although this was found to have no additional effect on superoxide production or chemotaxis.”

“Incubation … dehydroascorbic acid , but not ascorbate, resulted …) accumulation of intracellular ascorbate that was blocked by the glucose transporter inhibitor, cytochalasin B, and (ii) subsequent release of micromolar concentrations of ascorbate … external medium via a route that was sensitive to the anion channel inhibitor, 4,4′-diisothiocyanatostilbene-2,2′-disulfonate.”

“Loading cells to ascorbate concentrations of 0.3-1.6 mM generated intracellular DHA concentrations of 20-30 microM, indicative of oxidant stress in culture.”

“Incubation with ascorbate or DHAA raised intracellular ascorbate concentrations and mitigated the effects of septic insult.”

“The enhanced rate of ascorbate production from DHAA may protect these organs against the reactive oxygen species produced by subsequent, potentially lethal challenges.”

“This result points to the importance of the dehydroascorbic acid reductase in maintaining a high concentration of ascorbate in the cell.”

“The oxidation state of the dehydroascorbic acid/ascorbate couple increased concomitantly with the decrease in ascorbate concentrations early in infection and remained elevated throughout the infection.”

“Isoascorbic and dehydroascorbic acids inhibited ascorbate uptake (34 and 13 mmol/L, respectively), whereas high concentrations of glucose showed some stimulation.”

“First, preadsorption of the ascorbate antisera with ascorbic acid or dehydroascorbic acid resulted in the reduction of the labeling to background levels.”