Possible Interaction: Dactinomycin and Progesterone

“ Progesterone production was inhibited by actinomycin D in the hCG-pretreated cells, supporting the proposal that maintaining StAR protein synthesis is required for optimal steroid production in MA-10 mouse Leydig tumor cells.”

“The addition of cycloheximide or actinomycin D to the culture media inhibited the increase in tissue glycogen caused by progesterone.”

“Pretreatment with actinomycin D or cycloheximide clearly abolished the neuroprotective effects of progesterone and testosterone, while actinomycin D and cycloheximide did not show any effect on neuroprotection mediated by 17β-estradiol.”

“ DACT (135 mg/kg) increased … (2.9-fold) and progesterone (1.9-fold) above vehicle control concentrations, but the magnitude of the responses was much lower than that observed for … of ATR (200 mg/kg; 7.0, 9.0 and 11.0-fold above ACTH, CORT, progesterone controls, respectively).”

“Detachment of heterochromatin from the nuclear membrane was slightly delayed by actinomycin D , when given before progesterone.”

“On the other hand, actinomycin D enhanced the formation of progesterone induced secretory granules if it was administered after the steroid.”

“There was marked nuclear shrinkage as well as a significant decrease in the amount of nuclear pores by 2h after actinomycin D , administered either before or after progesterone.”

“There were significant changes in nuclear morphology after actinomycin D and progesterone injections.”

“When actinomycin D was given half an hour before progesterone , it inhibited the formation of secretory granules, whereas it could not prevent the formation of the organelles involved in protein synthesis (polysomes, rough endoplasmic reticulum, Golgi apparatus) and the increase in the amount of mitochondria.”

“With prior actinomycin D administration, the amount of nucleoli per nucleus was decreased 7h after progesterone , whereas their amount increased within 2h if the actinomycin D treatment followed progesterone injection.”

“The maturation of follicle-enclosed common frog oocytes stimulated by low progesterone concentration is inhibited by actinomycin D (5 μg/ml).The concentrations of progesterone, at which oocyte maturation was inhibited by actinomycin D, varied with the season and were different in different females.”

“ Progesterone decreased TH activity after pretreatment of the hypothalamic cells for 2 or 24 h with actinomycin D , an RNA synthesis inhibitor, suggesting that increased transcription does not mediate the effect.”

“The PROG effects were blocked by Actinomycin D and by RU 486.”

“The progesterone receptor antagonist RU486 and the transcriptional inhibitor actinomycin D blocked increases in PARP in ovarian surface epithelial cells incubated with progesterone.”

“Treatment with actinomycin D (5 micrograms/ml) reliably decreased the content of progesterone and increased that of testosterone.”