Possible Interaction: Dactinomycin and Polypeptides

“ Actinomycin D prevented the appearance of these polypeptide bands , suggesting that the heat-shock proteins in T. cruzi epimastigotes are induced at the level of transcription.”

“The synthesis of these polypeptides increases 10-fold during the first 2 h. Cycloheximide superinduces the 45 K polypeptides and actinomycin D (actD) blocks completely their induction by serum.”

“ Actinomycin D reduced the synthesis of virus, virus RNA, and virus polypeptides and also reduced the proportion of virus particles to empty capsids in the virus yields.”

“That these are host polypeptides encoded in cellular mRNAs has been shown by the inhibition of their synthesis by actinomycin D and by the similarity of the peptide maps of them and of polypeptides with the same electrophoretic mobility from uninfected cells.”

“ Polypeptides reduce the number of binding sites on DNA available for actinomycin D binding.”

“ Actinomycin D inhibited synthesis of the membrane polypeptides if added at the beginning of a 2 h dark preincubation, but not when added near the end.”

“… beta polypeptides normally occurring after … presence of actinomycin D than in its absence, whereas ongoing synthesis of gamma polypeptides at this time was rapidly reduced by actinomycin D. (iv) …) did not prevent the synthesis of alpha, beta, or gamma polypeptides, but did reduce the amounts of gamma polypeptides made.”

“… polypeptides normally occurring after … the presence of actinomycin D than in its absence, whereas ongoing synthesis of γ polypeptides at this time was rapidly reduced by actinomycin D. (iv) …) did not prevent the synthesis of α, β, or γ polypeptides, but did reduce the amounts of γ polypeptides made.”