Possible Interaction: Dactinomycin and Leucine

“ Actinomycin D inhibited activation of lysosomal cathepsins A, B, C, D, H and L in peritoneal macrophages, caused by T-dependent antigen (sheep erythrocytes), of mice CBA X C57BL/6 strain.”

“When actinomycin D injection preceded the [3H]leucine administration, all nucleoli decreased markedly and the grain counts increased by about 30% in all epithelial levels.”

“ Actinomycin D inhibited choleragen-stimulated incorporation of [3H]uridine into RNA of freshly prepared cells by 65% and reduced both RNA synthesis and incorporation of [3H]leucine into protein at 20 and 48 h of culture by approximately 90%.”

“Addition of actinomycin D to the washed cells caused a small increase in the rate of loss of capacity to decarboxylate tyrosine and leucine.”

“The addition of cycloheximide or actinomycin D inhibited the incorporation of [3H]leucine into TCA-precipitable proteins and prevented gap junction formation.”

“Addition of actinomycin D or cycloheximide along with growth hormone caused the accelerated rate of leucine consumption to persist through at least the fourth hour.”

“Autoradiographs and controls indicated that actinomycin D prevented uridine incorporation by about 90% but prevented leucine and thymidine incorporation by 6% or less in these cells.”

“ Actinomycin D at a concentration of 10 μg/ml gave substantial reduction of incorporation of both tritiated uridine and leucine into incubated erythrocytes, suggesting a continued dependence on RNA synthesis for the residual protein synthesis occurring in these cells.”

“ Actinomycin D , at doses (25 and 50 μg/ml) that block RNA synthesis to less than 3% of the control rate, inhibits the incorporation of [3H]leucine into adenohypophyseal proteins and the release of newly synthesized proteins by 50 and 60%, respectively, of the control rates.”

“The technique has been applied to determining rates of protein synthesis in a protein-secreting …, the galea of the silkmoth, Antheraea polyphemus, after progressive exposure to actinomycin D. Actinomycin D is shown to "…" incorporation of [3H]leucine in the differentiation-specific product of the galea, the zymogen of cocoonase.”