Possible Interaction: Dactinomycin and Calcium Supplement

“Both cycloheximide and actinomycin D inhibited CA 125 synthesis.”

“Increasing the calcium concentration to 1.2 mM resulted in a 6-fold increase in CaBP9K mRNA after 3 h and … increase in CaBP9K protein content after 24 h. Calcium may increase CaBP9K gene transcription, since simultaneous addition of 1 microM actinomycin D … calcium blocked the calcium … CaBP9K mRNA.”

“ Actinomycin D (1.6 microM, 12 h) was also effective in inhibiting 1,25-dihydroxy-vitamin D3-dependent Ca uptake in myoblasts.”

“Also, actinomycin D was shown to block both 1,25-dihydroxy-cholecalciferol stimulated Ca 2 + transport and RNA synthesis.”

“Thus the vitamin D-stimulated absorption of Ca is inhibited by actinomycin D (ref.”

“ Actinomycin D … calcium across the mucosal surface of vitamin D-deficient as well as vitamin D-treated rats so that its action is on the calcium … 3 hours of its subcutaneous injection and is less marked at 18 hours which suggests a direct interaction with the calcium transport system.”

“The incubation of femora removed from both intact and parathyroidectomized animals resulted in media Ca levels that were significantly lower after 4 hours incubation when AMD was added in vitro .”

“Injection of actinomycin D into rats completely prevents both the rise in serum calcium normally induced by vitamin D and the increased transport of calcium by everted intestinal sacs.”