Possible Interaction: Cysteine and Nitroprusside

“Thiol compounds such as glutathione, cysteine , and cysteamine reduce NP to generate NP nitroxide radical anion and a paramagnetic species characterized as a dithiolated dinitroso iron complex (DDIC), Fe(NO)2(RS)2 g = 2.030, A(14N) = 2.2 G(2N), and A(1H) = 1.1 G(4H).”

“Addition of hemoglobin, L-cysteine , FeSO4 and L-cysteine with SNP led to a decrease of the inhibition from (85.40+/-2.90)% to (5.90+/-2.90)%, (25.86+/-4.02)%, (30.16+/-2.75)%, (16.71+/-2.30)%, respectively.”

“However, while L-cysteine reduced the effect of AS, it increased the effect of SNP on this parameter.”

“ Cysteine (1 mM) enhanced cyclic GMP formation induced by sodium nitroprusside and nitroglycerin.”

“There was a positive linear correlation between the worm mortality … 0.02-1.00 mmol/L. Combination of hemoglobin, L-cyst , FeSO4 and …/L SNP led to a decrease of the mortality from (80.8 +/- 1.1)% ….8 +/- 2.3)%, (74.8 +/- 2.4)%, (72.7 +/- 5.6)%, respectively.”

“Relaxations produced by exogenous NO• (0.25–2.5 μM) were markedly prolonged and relaxations produced by sodium nitroprusside (0.001–0.3 μM) were enhanced by 1 and 3 mM L‐cysteine.”

“Serosal application of the NO-donating compounds sodium nitroprusside or isosorbide dinitrate (in the presence of 5 microM L-cysteine ) resulted in concentration-dependent increases in the short circuit current.”

“In addition, it was seen that the withdrawal of nitric oxide stocks carried out by l-cysteine is capable of being reversed with the use of the SNP.”

“Increased rates of oxidation of dihydrorhodamine-123 were also observed during the reduction of SNP with either L-cysteine or glutathione.”

“This method has been used to measure nitric oxide formation during the incubation of glyceryl trinitrate or sodium nitroprusside in the presence of cysteine.”