Possible Interaction: Cysteine and Buthionine Sulfoximine

“Exposure to buthionine sulfoximine inhibits growth, prevents GSH synthesis, and results in accumulation of total cysteine , protein‐bound cysteine, and protein disulfides.”

“Other findings supporting this hypothesis were changes in sperm functionality after BSO … and changes in GSH and GSSG validated using HPLC-MS, showing that BSO prevented the increase in … in the presence of cysteine , although important stallion to stallion variability occurred and … differences in expression of glutamate cysteine ligase.”

“Urine excretion of cysteine and its precursors was increased by BSO.”

“ BSO reduced cysteine and GSH levels in all birds, but improved phenotypes (bibs larger than controls) were only expressed by high‐quality birds (BSO birds with largest bibs initially).”

“Furthermore, the toxicity of both silver types was generally decreased in cells treated with cysteine while treatment with buthionine sulfoximine increased the toxicity of both silver types.”

“In the YSF, BSO caused a significant increase in total Cys concentrations to 1.7 mM but did not significantly change the E(h) for Cys/CySS.”

“ Buthioninesulfoximine administration depressed the elevation of S-adenosylmethionine, but increased the catabolism of cysteine to taurine.”

“ BSO significantly decreased plasma GSH and GSSG, oxidized GSH/GSSG E(h), and increased plasma Cys and CySS concentrations.”

“ BSO treatment resulted in a significantly increased frequency of DNA deletions and decreased concentrations of GSH and cysteine.”

“Both 2-ME and l-cysteine also reversed the BSO inhibition of T cell proliferation.”

“Intracellular l-cysteine levels were reduced with BSO treatment and restored with cotreatment with NAC or l-cysteine.”