Possible Interaction: Copper and Phospholipids

“Mild oxidation (< 6h) of human high density lipoprotein (HDL) induced by incubation with copper ions increased its thiobarbituric acid reactivity, decreased its phospholipid content, increased its free cholesterol to phospholipid ratio and reduced its ability to mediate cholesterol efflux from cells.”

“These experiments suggest that the primary toxic effect of copper is mediated through its oxidant actions on membrane phospholipids rather than through its potential inhibitory effects on intracellular enzymes.”

“In particular, the analysis showed that Cu had a significant negative effect on the polyunsaturated PLFA , linoleic acid (18:2omega6,9), which has previously been reported to correlate positively (R2 = 0.92) with freeze tolerance in D. octaedra.”

“It was demonstrated that interaction of copper ions with these membrane stimulated the process of lipid peroxidation and caused changes in the transbilayer distribution of aminophospholipids.”

“The thiobarbituric acid reactive substances assay reveals that Cu ions released from the surface of Cu, Cu-SiC FGC triggers the oxidative degeneration of phospholipids (most abundant constituent of bacterial cell membrane).”

“The total PLFA concentration was reduced by SDZ and/or Cu , which resulted from reduced bacterial and actinomycetic biomass.”

“In comparison with the control, only the excess of Cu caused a decrease in the lipid to protein ratio as well as a change in the phospholipid composition , with a lower phosphatidylcholine to phosphatidylethanolamine ratio.”