Possible Interaction: Copper and Histidine

“Higher HIs were found at the locations in close vicinity to copper mining and processing units compared to other locations.”

“The affinity of both histidines for copper is much lower than that of the surface histidines residues, and they are inaccessible for modification with chemical reagents.”

“That the enzyme is inactive when these histidines are either bound with copper or when replaced with serines suggests that these histidines perform a catalytic function.”

“ Copper ions , although more stimulatory towards fluorescent lipid complex formation than iron ions, do not stimulate lipid peroxidation when tightly bound at the active centre of proteins, but are reactive when loosely bound to albumin and histidine.”

“In the presence of low concentrations of histidine (0.5-2.0 mM), which forms high affinity complexes with copper , the rate of ascorbate oxidation was similar to that recorded in its absence.”

“Upon the addition of histidine, the fluorescence intensity of CP@Cu exhibits a linear correlation with the concentration of histidine ranging from 0-200μM (correlation coefficient R(2)=0.9912).”

“While the structure of the apo state is greatly influenced by the interaction between the rings in the histidine , tryptophan and proline residues, the region shows evidence of highly ordered coordination sites in the presence of copper ions.”

“Furthermore, the complexes of histidine and copper inhibited the formation of peroxidized lipids in an in vitro assay.”

“Induction of apoptosis by HPH‐3 was inhibited by zinc and copper ions , indicating that chelation with ferrous ion is responsible for induction of apoptosis, as is the case in chelation by bleomycin to cleave DNA.”

“These results suggest that, in SHS, Cu absorbed in the presence of l-histidine is in an abnormal complex involving albumin, which does not allow for holoceruloplasmin biosynthesis.”