Possible Interaction: Copper and Ascorbate

“On the contrary, the loss of ascorbate and the levels of Asc.- were significantly increased by the addition of physiologically relevant concentrations (1 microM) of copper ions (Cu2+ but especially Cu+).”

“La3'-nucleotidase activity was found to be resistant to ROS generated during oxidation reactions of ascorbate and hydrogen peroxide catalyzed by copper.”

“By fluorometry using a fluorescent Cu(II) chelator and by centrifugal ultrafiltration, we have studied copper binding of the whole cytosolic proteins from mouse brain and liver, and found that their binding capacity and affinity for copper were markedly increased by ascorbate.”

“Although urate, xanthine, and ascorbate can protect superoxide dismutase activity of Cu ,Zn SOD from inactivation, they were not effective at protecting Cu,E SOD.”

“The cytotoxic activity of SBA and ascorbate was significantly enhanced in the presence of copper and the stimulation effect of copper was reduced by a heavy metal antagonist.”

“ Ascorbate at physiological levels and above impedes the intracellular binding of copper to Cu,Zn superoxide dismutase.”

“ Ascorbate has been known to antagonize the intestinal absorption of copper.”

“Synergism between copper and ascorbate was better demonstrated following the treatment of infected erythrocytes: while ascorbate alone supported parasite development and copper alone had only a marginal suppressive effect, the combination of copper and ascorbate yielded a marked inhibition of parasite growth.”

“The combination of copper and ascorbate arrested parasite maturation, an effect which was more pronounced in G6PD(-) cells.”

“ Copper (Cu2+), when added with the ascorbate solution at 5 micrograms/ml (0.022 mM), exhibited a catalytic effect on the inactivation of these viruses.”

“The antitumor activity of ascorbate can be greatly potentiated by the combination with copper ions or copper chelates.”

“While the exposure of the enzyme to either ascorbate or copper did not cause enzymatic inactivation, the incubation of the enzyme with a combination of both ascorbate and copper resulted in a loss in acetylcholine esterase activity, which was time dependent.”