Possible Interaction: Chymotrypsin and Trypsin

“Numerous clinical studies were published … indicated that intramuscularly administered trypsin … chymotrypsin had a favorable influence on the inflammatory process in thrombophlebitis.l3 Among these studies was a double-blind investigation by Kryle et a1.,4 showing excellent results in 28 of 39 thrombophlebitis patients treated with trypsin i.m.”

“ Chymotrypsin , another acinar protease, has recently been shown be play critical role in clearance of pathologically activated trypsin protecting against pancreatic injury.”

“The system developed promoted an impressive protection of INS (80%) after 120 min of incubation with trypsin and alpha‐chymotrypsin , while only 3% of free INS remained intact after the same time, possibility due to the calcium chelating activity of the polymers in inhibiting the proteolytic activity.”

“When vesicle-coated microspheres were treated with trypsin and chymotrypsin or heated, this resulted in a significant loss of attachment, suggesting a possible involvement of proteinaceous molecules in the process.”

“But, whatever the species, chymotrypsin is inhibited more quickly than trypsin.”

“While very few of the compounds were selective for KLK5, the selective inhibition of trypsin against chymotrypsin was achieved.”

“The combination of trypsin and chymotrypsin produced the single-missed cleaved peptide LQQC*PFEDHVK in high yield.”

“ Trypsin then activates other pancreatic proenzymes such as proelastase, chymotrypsinigen , procarboxypeptidase, and phospholipase A2 initiating autodigestion, as well as proinflammatory complement and kinin cascade systems.”

“These observations suggest that trypsin and chymotrypsin are involved in feedback regulation of pancreatic secretion in man which is, however, not mediated by CCK or secretin.”

“In spite of this, these can bind trypsin and chymotrypsin , showing that the N-terminal pentapeptide is not indispensable for inhibition function.”