Possible Interaction: Chymotrypsin and Leucine

“… Leu … chymotrypsin in the buffer and tissue, as well as the total activity (P < 0.05), especially at 0.40 and 0.80 mM. Compared with … had no effect on the tissue concentration of α-amylase, trypsin, and chymotrypsin or the total activity of α-amylase (P > 0.05).”

“For example, free leucine supplementation increased the amount of phosphorylation of S6 and 4EBP1 proteins and at the same time generated a decrease in chymotrypsin- and trypsin-like activities of the 26S proteasome associated with a P-FOXO3a/FOXO3a ratio in favour of antiproteolytic processes.”

“At the normally reported pH optimum (pH 7-8) of CHYM and SGPB, the substrate with Leu at the reactive site is more than 25,000-fold more reactive than that with Asp.”

“The difference indicates that removal of leucine from the second aminoacyl site S2 of chymotrypsin weakens the LBHB and decreases the basicity of the H57-D102 diad relative to the case in which S2 is occupied by leucine.”

“ L-leucine caused a significant (p < 0.05) alteration of oxidative-elevated acetylcholinesterase and chymotrypsin activities , while concomitantly elevating the activities of ATPase, ENTPDase and 5'-nucleotidase.”