Possible Interaction: Chymotrypsin and Isoflurophate

“Photo-inactivation of chymotrypsin eliminated the reactive site for the attachement of diisopropyl fluorophosphate.”

“Previous communications from this … that the inhibition of cy-chymotrypsin … diisopropyl fluorophosphate (DFP) is a stoichiometric reaction that comprises the introduction of a single diisopropyl phosphate group into the molecule of …, the elimination of fluorine as HF, and the production of an undenatured, crystallizable, inhibited enzyme protein (l-3).”

“The chymotrypsin was inactivated by DFP , and was inhibited by chymostatin and the chymotrypsin inhibitor, POT-1.”

“To firmly establish that the results are … particular enzymes, we show that the chymotrypsin … diisopropylfluorophosphate and not RNAasin, while the reverse is true for RNAase A. We further show that the differential … the association of a proteinase-resistant, heat-stable low molecular weight factor with the unactivated glucocorticoid receptor.”

“The concentration of DFP employed was sufficient to have inhibited the esterolytic activity of proteases such as trypsin or chymotrypsin , if present.”

“Since DFP reversed the inhibition by both chymotrypsin and mast cell granules, it was concluded that granule-bound, mast cell chymase was responsible for the inhibition of lipase activity by mast cell granules.”

“Diisopropyl fluorophosphate DFP ) is a highly specific inactivator of e&erases, such as cholinesterase and chymotrypsin (2), and combines irreversibly with these enzymes (3,4).”

“The rate of inactivation with DFP was much faster than that for typsin [EC 3.4.21.4] and chymotrypsin [EC 3.4.21.1.],”

“In another procedure the enzyme is treated with diisopropyl fluorophosphate in an amount designed to inhibit all of the chymotrypsin but only partially inactivate the trypsin (7).”

“IN a recent article Stein and Laidler1 described the inhibition of chymotrypsin by diisopropyl phosphorofluoridate (DFP).”