Possible Interaction: Chymotrypsin and Insulin

“The system developed promoted an impressive protection of INS (80%) after 120 min of incubation with trypsin and alpha‐chymotrypsin , while only 3% of free INS remained intact after the same time, possibility due to the calcium chelating activity of the polymers in inhibiting the proteolytic activity.”

“ Chymotrypsin was significantly associated with insulin in the two adjusted models (p = 0.005; p = 0.003) and just missed statistical significance in the unadjusted model (p = 0.066).”

“For stability and permeation of insulin in the presence of α‐chymotrypsin , an enzyme‐to‐inhibitor ratio of 1:1 and 1:2 was used.”

“The flux enhancement of insulin in the presence of α‐chymotrypsin and DkOVM is encouraging.”

“This was in agreement with the 2‐fold increase in flux values of insulin in the presence of α‐chymotrypsin and DkOVM at the 1:2 ratio of enzyme to inhibitor.”

“Finally, we observed that Fao cells … of a covalent inhibitor of chymotrypsin after … insulin for 10 h. We propose that a chymotrypsin-like activity is involved in the intracellular signalling leading to … hepatoma cells up to a non-return point situated in the middle of G1 (6-8 h).”

“In summary, these results show that chymotrypsin substrate analogues efficiently inhibit the internalization of insulin and insulin receptors in adipocytes and implicate a possible role for endogenous chymotrypsin-like enzyme(s) or related substances in receptor-mediated endocytosis of insulin.”

“The acceleration of glucose oxidation by insulin was also reduced by prior incubation of cells with chymotrypsin , papain, or ficin, but not with purified collagenases or an acidic proteinase of Clostridium histolyticum.”

“At a molar ratio of 172:1 (insulhr.enzyme), chymotrypsin caused near-total loss of insulin within 40 min, while very little insulin was degraded by trypsin.”