Possible Interaction: Chondroitin Sulfates and Hyaluronoglucosaminidase

“ Hyaluronidase treatment altered the immunostaining for chondroitin sulfate epitopes, but not for lubricin.”

“Enzymatic depletion of the HS and CS chains by heparinase and chondroitinase treatment decreased HS and CS expression but did not alter the expression of the HS core proteins perlecan and syndecan.”

“The titration readings are decreased by the action of hyaluronidase on chondroitinsulphate.”

“We have demonstrated the possibility of stimulation of the function of various types of precursor cells with hyaluronidase modified with chondroitin sulfate.”

“ Chondroitin sulfate is degraded by chondroitinase ABC to three primary unsaturated disaccharides, (1) 2-acetamido-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-…-galactose, and (3) 2-acetamido-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-6-O-sulfo-D-galactose, when treated with chondroitinase.”

“The number and size of aggregates were not affected by inclusion of chondroitin sulphate or fibronectin in the culture medium, but both increased in the presence of hyaluronidase.”

“Additionally, we found that hyaluronidase SD can specifically and endolytically cleave the internal unsulfated regions of chondroitin sulfate chains.”

“The results obtained with Sertoli cell monolayers show that the CS of the mixed PG is degraded by chondroitinase , while the CS-PG is not degraded.”

“For these experiments, epithelia were cultured in the presence of hyaluronidase , which degrades CS , and p-nitrophenyl beta-D-xyloside, which inhibits the formation of links between the core protein and glycosaminoglycan side chains in the PG; the absence of intact CS was confirmed by gel filtration.”

“The stability of the collagen in hyaluronidase-treated cartilage was found to be higher when measured in a solution of chondroitin sulphate (30 g/dl) than in buffer alone.”