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“Even the completely digested products of casein with trypsin or chymotrypsin showed inhibitory activities against the two proteases.”
“It was suggested that not only the intact casein but also the products digested with trypsin or chymotrypsin contribute to the inhibitory effect of casein on the proteases in the intestinal lumen.”
“Intraduodenal administration of casein in a dose of 400 mg/h caused significant increases in plasma CCK concentration and pancreatic secretion of juice volume and outputs of amylase and trypsin.”
The American journal of physiology • 1986 | View Paper
“ Trypsinization decreased the Km of phosphatase for casein from 10.8 to 1.2 microM and increased the Vmax from 4.9 to 30.9 nmol (mg of protein)-1 min-1.”
“It was found that conjugated trypsin required lower incubation periods for complete hydrolysis of casein , BSA & ovalbumin as compared to free enzyme.”