Possible Interaction: Carbachol and Tyrosine

“ Carbachol also increased tyrosine phosphorylation of p38 MAP kinase.”

“In this study, we show that in cells expressing ml mAChRs, the cholinergic agonist carbachol , induces a rapid and dose-dependent increase in tyrosine phosphorylation of cellular proteins which are different from those induced by PDGF.”

“ CCh rapidly induced tyrosine phosphorylation of FAK in T84 cells.”

“An investigation of tyrosine phosphorylation levels of the plasma membrane Ca2+-ATPase (PMCA) demonstrated that CCh stimulates an increase in tyrosine phosphorylation levels, which has been reported to inhibit Ca2+ pump activity, whereas in contrast, BK stimulates a reduction of PMCA tyrosine phosphorylation levels.”

“ CCh (100 μm) stimulated tyrosine phosphorylation and association of the Ca2+-dependent tyrosine kinase, PYK-2, with the EGFR, which was inhibited by the Ca2+ chelator, BAPTA (20 μm).”

“ CCh also induced tyrosine phosphorylation of p60 src and association of p60 src with both PYK-2 and the EGFR.”

“Activation of muscarinic receptors with carbachol was found to inhibit EGF-induced signaling, including tyrosine phosphorylation of the adaptor protein Cbl and of the EGF receptor, and complex formation between Shc proteins and the EGF receptor and Grb2.”

“That carbachol can activate src family kinases was indicated further by the finding that carbachol induced an increase in tyrosine phosphorylation of p120‐src substrate, which was inhibited by PP1.”

“ Carbachol concentration-dependently induced tyrosine phosphorylation of several proteins, including one of 42 kDa.”

“ Carbachol increased the phosphorylation of Pyk2 on tyrosine 402 and c-src on tyrosine 416 in a time-dependent manner.”

“ Carbachol also increased tyrosine phosphorylation of Pyk2, p60Src, and the epidermal growth factor receptor (EGFR).”