Possible Interaction: Carbachol and Phosphatidic Acid

“ PA potentiated the effect of carbachol on [Ca2+]i and Cl- secretion, although it did not stimulate Cl- secretion by itself.”

“Taken together, these results suggest that Ca2+ mobilization is involved in mAChR-mediated hydrolysis of PC by a … protein kinase C activation is required for mAChR-stimulated hydrolysis of PC by a phospholipase D. Both carbachol and PMA … formation of [3H]phosphatidic acid ([3H]PA) in …]myristate-labeled PC.”

“With carbachol , there was a 20-fold increase in the [3H]-MI incorporation rate into inositol phospholipids, decreases in PI and PIP2 contents, and increases in phosphatidic acid and diacylglycerol contents.”

“We now show, for the first time, that glucose and carbachol , at concentrations which stimulate insulin secretion, significantly increase endogenous phosphatidic acid levels in pancreatic islets by 2-fold at 1 min, nearly 3-fold at 2 min, and over 3-fold at 30 min compared to control.”

“ Carbachol (CCh, 10(-8)-10(-4) M) increased in concentration-dependent manner the mass of phosphatidic acid (PA), but not the mass of diacylglycerol (DG) in the Taenia coli from guinea pig.”

“Addition of carbachol (10 microM) to 32P-labeled tissue resulted in increased labeling of phosphatidic acid and hydrolysis of PIP2.”

“Stimulation of muscarinic receptor by carbachol (1 mM) resulted in a decrease in 32Pi incorporation into phosphatidylinositol-4,5-bisphophaphate (TPI) and phosphatidylinositol-4-phosphate (DPI), and an increase in 32Pi incorporation into phosphatidylinositol (PI) and phosphatidic acid (PA), whereas no significant effect on other membrane phospholipids was found.”

“ Carbachol induces a rapid reduction in the mass of phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-monophosphate and a rapid increase in the mass of 1,2-diacylglycerol and phosphatidic acid.”

“ Carbachol stimulated phosphatidic acid synthesis in cholinergically enriched synaptosomes from rat cerebral cortex.”

“The muscarinic agonists, carbachol and pilocarpine, stimulated 32Pi-labelling of phosphatidylinositol (PI) and phosphatidic acid (PA) in both iris and ciliary process.”