Possible Interaction: Carbachol and Methionine

“In atrial myocytes, CCh induced an inwardly rectifying K+ current, IK,CCh, with an EC50 value of 3-4.5·10-7 M and inhibited Ca2+ current (ICa) by 28 ± 8% and 51 ± 6% at 10-7 M and 10-6 M, respectively.”

“Compared with 4-month-… somatostatin release by carbachol was less in … 10−8 and 10−7 M , and less in 12-month-old rats at 10−7 M. The decreased basal gastrin secretion … were further confirmed in conscious aged rats tested by means of oral gavage with 10% peptone.”

“Stimulation of muscarinic cholinergic receptors with carbamylcholine (2× 10−5 M ) caused a net release of K+ from sublingual slices incubated in an enriched, oxygenated Krebs Ringer bicarbonate medium of 35±7% after 10 min of incubation.”

“ Carbachol caused a slight but significant increase in uptake of22Na by the cells in the presence or absence of ouabain ( 10−3 M).”

“The effects of carbachol were blocked by 10−5 M atropine.”

“Exposure to 10−4 M carbachol during 4 h almost doubled the control activity.”

“α‐Bungarotoxin (10−7 M), decamethonium 10−5 M ), d‐tubocurarine (5 × 10−5 M), and strychnine (5 × 10−5 M) blocked the carbachol depolarization by about 90%.”

“A 15% decrease in beating rate occurred in cells exposed to 10–3 M carbamylcholine.”

“In cells labelled to equilibrium with 42K+, exposure to 10–3 M carbamycholine caused a 33% increase in the rate of 42K+ efflux from the cell with a half-maximal effect at a carbamylcholine concentration of 8×10–5 M. Steady state Intracellular K+ content remained at control levels.”

“The magnitude of the response was dependent on the extent of receptor occupancy and the response elicited by a saturating dose of carbamylcholine was blocked completely by 10−7 M at‐ropine, a specific muscarinic antagonist.”

“ Carbachol stimulated secretion to 131% of controls at 5 × 10−5 M and to 115% at 5 × 10−6 M but had no effect at 5 × 10−7 or 5 × 10−8 M. The secretory response was blocked by the respective β‐adrenergic and cholinergic antagonists, propranolol and atropine.”

“ Carbachol at 10 −5 M decreases the rate at which contractions occur and also decreases the complexity of each contraction burst and at 10 −4 M completely suppresses activity.”