Possible Interaction: Carbachol and Glucose

“ Glucose , which augmented [Ca2+]er by about 25%, potentiated the Ca2+-mobilizing effect of carbachol , explaining the corresponding observation in cytosolic [Ca2+].”

“Incubation …/l glucose resulted in a two- to fourfold increase in human IAPP-LI, mouse IAPP-LI, and IRI secretion compared with 1.67 … mmol/l glucose and 10 mmol/l arginine, 0.1 mmol/l 3-isobutyl-1-methylxanthine (IBMX), and 5 micromol/l carbachol induced a >… peptides.”

“Similarly, perifusion with carbachol or exendin-4 (Byetta) with glucose (6 mM) resulted in 1.32- and 1.35-fold increase in insulin secretion above basal.”

“Second, over 15 min exposure to carbamylcholine (0.1 mM), in the presence of d-glucose , the cytosolic concentration of Ca2+ is increased to a greater relative extent in dispersed islet cells from ω3 rats, as compared to control animals.”

“To stimulate ion transport, we found that increases in short circuit current induced by carbachol and glucose were higher in the total parenteral nutrition group compared with the control group.”

“The addition of 5 microM carbachol , 500 nM forskolin or 20 mM KCl to mouse islets together with 20 mM glucose resulted in a dramatic augmentation of insulin output.”

“Previously, we have shown in normal dogs that, after intracerebroventricular (ICV) administration of carbachol (a model of moderate stress), increases in both the metabolic clearance rate (MCR) of glucose and endogenous glucose production (GP) occur.”

“Compared with responses observed from control islets incubated for 3.5 ….6 mmol/L glucose alone, prior exposure to 10 mmol/L glucose , 20 mmol/L … 10 micromol/L carbachol reduced peak second-phase insulin release rates to a subsequent …/L glucose stimulus by 63%, 81%, or 70%, respectively.”

“All three parameters are increased synergistically when glucose and CCh are combined.”

“Surprisingly, carbachol added prior to, or simultaneously with, glucose attenuated nutrient activation of CaM kinase II.”