Possible Interaction: Caffeine and Procaine

“The cell vacuolisation cannot be prevented by 3-methyadenine, brefeldine A, and cytochalasine D. On the other hand, nocodazole and caffeine prevent cytoplasm vacuolisation induced by procaine.”

“The source of this Ca2+ is tentatively identified as the sarcoplasmic reticulum (SR) for the following reasons: (1) prior exposure to 20mm caffeine depleted this Ca2+ store, (2) procaine (10mm) inhibited the response, and (3) the extracellular space or “clefts” and the mitochondria could be eliminated as possible sources.”

“Lower concentrations of tetracaine (0.5mm) and procaine (⩽10mm) blocked contractions due to caffeine (at pCa ⩾6.73), sulphydryl oxidizing agents, or Ca2+-induced Ca2+ release (CICR).”

“The pretreatment of fibres with procaine prevents both the generation of caffeine contractures and the occurrence of changes in the ultrastructure.”

“The response to 25 mM caffeine was only moderately inhibited by 25 mM procaine , and almost completely abolished by 50 mM procaine and 100 microM ryanodine.”

“(45)Ca(2+) release induced by caffeine was completely inhibited by typical blockers of Ca(2+)-induced Ca(2+) release, such as Mg(2+), ruthenium red, or procaine.”

“ Procaine (1 mM) inhibited the transient contraction induced by caffeine (10 mM) in Ca2+-free Krebs solution.”

“ Procaine , an antagonist of ryanodine receptor, selectively blocked the effect of caffeine but not the effect of InsP3.”

“On the other hand, procaine inhibited the responses to caffeine , but not those to IP3.”

“Surprisingly, procaine had only a minor effect on tension transients elicited by Cl− and caffeine together.”