Possible Interaction: Caffeine and Heparin

“The effect of caffeine was attenuated by exposure to the ryanodine receptor blocker dantrolene or when the membrane‐impermeant inositol trisphosphate receptor antagonist, heparin , was entrapped into synaptosomes; the combined treatment with dantrolene and heparin abolished the release elicited by caffeine.”

“2‐Aminoethoxydiphenyl borate and heparin also increased the response to caffeine.”

“Intracellular application of heparin (5 mg ml–1) abolished the response to carbachol but potentiated that to caffeine.”

“However, in the presence of caffeine, heparin decreased maximal Ca2(+)-activated tension in both muscles.”

“Analysis of cells in media with altered composition indicated that the inclusion of either heparin or caffeine significantly promoted capacitation to about the same extent, but together, heparin plus caffeine had an even more stimulatory effect.”

“The synergistic effect of caffeine and heparin was observed in the penetration rate of fresh mature oocytes but not in the stored oocytes, indicating the difficulty of assessing sperm capacitation and/or acrosome reaction of salt-stored mature bovine oocytes under the present condition.”

“When heparin was added to the caffeine in the fertilization medium, the penetration rate of oocytes increased significantly to 68% (P < 0.001), indicating that both chemicals act sinergistically to induce capacitation and/or acrosome reaction of spermatozoa and stimulate in vitro fertilization of cattle oocytes.”