Possible Interaction: Caffeine and Aphidicolin

“…,caffeine , which is a well-known inhibitor of DNA damage checkpoint kinases, … ligase I in MiaPaCa cells treated with ara-C and aphidicolin , suggesting that caffeine-sensitive kinases might be important mediators in the pathway leading … ligase I levels in response to anticancer drugs, including ara-C and aphidicolin.”

“Flow microfluorometric analysis showed that caffeine , partially or fully, abrogates the cell cycle progression block produced by aphidicolin.”

“In aphidicolin-treated V79 cells, the increase in the dCTP level due to exogenous cytidine was almost completely blocked; caffeine also substantially overcame this effect of aphidicolin.”

“Increased cell growth is also observed in cultures treated with both aphidicolin and caffeine compared to cultures treated with aphidicolin only.”

“Microscopic examination of V79 cultures treated with aphidicolin for 8 h showed a marked reduction in the frequency of round mitotic cells, as is expected from a drug which inhibits progression through the cell cycle by inhibiting DNA replication; this effect of aphidicolin was also reduced by caffeine.”

“The division arrest induced by APH was partly overridden by caffeine (CAF) treatment, which caused the fragmentation ("pulverization") of the chromosomes in G2 micronuclei.”