Possible Interaction: Busulfan and Glutathione

“The finding of an electrophilic metabolite of GSH suggests that alteration of cellular GSH concentrations, irreversible nonreducible glutathionylation of proteins, and interference with GST function may contribute to the toxicity of busulfan.”

“The alkylating capacity of busulfan is compromised in vivo by enzymatic conjugation with glutathione , principally catalysed by glutathione S‐transferase alpha (GST alpha).”

“During high-dose therapy, busulfan may deplete hepatocellular levels of GSH.”

“2) In GSH-depleted hepatocytes: busulfan profoundly depletes GSH ; toxicity is mediated by oxidative stress and is prevented by restoring GSH levels; cell death may be due to unopposed endogenous oxidative stress.”

“ Busulfan given in vivo or in vitro decreased hepatocyte glutathione ( GSH ) by 60 and 50%, respectively.”

“These results affirm that busulfan is capable of evoking a response from the enzymes involved in the various pathways of GSH enabling the lens to prolong its clarity.”

“Based on theoretical background, the application of Bu prior to Cy decreases the levels of glutathione in hepatocytes; therefore, the metabolism of Cy is hampered and exacerbates liver toxicity [4].”

“ Busulfan , depletes glutathione level in liver and causes elevated levels of CY metabolites.”