Possible Interaction: Bradykinin and Genistein

“In addition, genistein promoted an increased response to a third exposure to the agonist when applied after washing the cells that had been previously challenged with two increasing doses of bradykinin.”

“Our results suggest that the down-regulation of ACE with a consequent change in the circulating levels of angiotensin II (Ang II), vasorelaxant angiotensin-(1-7) [Ang-(1-7)] and bradykinin plays an important role in cardiovascular effects of genistein through the ERK1/2 pathway.”

“On the other hand, genistein (a tyrosine kinase inhibitor) was able to inhibit thapsigargin (3.12 ± 0.22, n = 5, vs. 1.28 ± 0.16, n = 4, p < 0.001) as well as BK responses (6.46 ± 0.66 vs. 2.89 ± 0.61, n = 4, p < 0.05).”

“Tyrphostine A23 and genistein (tyrosine kinase inhibitors) decreased the bradykinin (10 n M)-induced IP3 production potentiated by 2 n M insulin from 400.7 ± 19.4 pmol/mg protein to 297.3 ± 42.4 and 314.3 ± 37.5 pmol/mg protein, respectively.”

“ Genistein and tyrphostin A-23, tyrosine kinase inhibitors, completely reversed the elevated IP3 formation by BK and insulin.”

“ Genistein , a specific tyrosine kinase inhibitor, prevented the phosphorylation of ERK2 by BK.”

“ Genistein , an inhibitor of tyrosine kinase, did not affect baseline diameter of the basilar artery but inhibited vasodilatation in response to acetylcholine and bradykinin , without affecting vasodilatation produced by sodium nitroprusside.”

“ Bradykinin- and InsP4-induced Ca2+ influx was blocked by 10-100 microM genistein , a tyrosine kinase inhibitor.”

“In addition, genistein partially inhibited BK stimulation of inositol phosphate formation.”

“ Genistein , a specific tyrosine kinase inhibitor, prevented the phosphorylation of ERK2 by BK.”