Possible Interaction: Bleomycin and Copper

“The Cu concentrations in conditioned media of cultured HLF cells exposed to BLM (10 and 30 μg/mL) were increased significantly to 1.48 and 2.46-fold of the control, respectively.”

“Correlatively bleomycin decreased the copper associated enzyme tyrosinase, increased the zinc dependent lactate dehydrogenase (LDH) and did not affect the manganese dependent glutamine synthetase.”

“The inhibition mechanism is … to chelating action of bleomycin toward … copper atom at the active site of the enzyme together with some other kinds of binding, for the addition of the … ions or extensive dialysis completely reversed the inhibition and bleomycin Cu(II)-complex did not inhibit the enzyme.”

“Additionally, the US Environmental Protection Agency has published guidance on the use of a BLM to develop water quality criteria for Cu.”

“DNA strand scission by bleomycin in the presence of Cu and Fe was further characterized.”

“Remarkably, it was found that the extent of DNA degradation obtained with bleomycin in the presence of Fe and Cu was greater than that obtained with either metal ion alone.”

“ Bleomycin is inactivated by copper and zinc ions, probably by chelate formation, which might cause a contortion of the antibiotic.”

“CuSO 4 (but not Co, Mn, Zn or Mg salts) with NADPH nicks DNA by the superoxide pathway but is inhibited by bleomycin which sequesters the Cu 2 + and prevents its reduction.”

“The ease of formation of the copper or cobalt complexes is suggested as a possible mechanism for the inhibition of the attack of DNA by bleomycins.”