“By in situ simultaneously fluorescent monitoring, Ca2+ … (30 microM), ryanodine (50 microM), and nicotinamide (… BK (1 microM)-induced increase in intracellular [Ca2+] by 78%, 80%, and 74%, respectively, whereas these compounds significantly … blockade with 2-aminethoxydiphenyl borate (50 microM) only blunted BK-induced Ca2+-NO signaling by about 30%.”
“Furthermore, HPLC analysis showed that the conversion rate of beta-nicotinamide guanine dinucleotide into cyclic GDP ribose dramatically increased by stimulation with BK , which was blockable by nicotinamide.”