Possible Interaction: Aspirin and Serum Albumin, Human

“Preincubation of HSA with acetylsalicylic acid (≈ 11 mM) and glucuronic acid (50 mM) decreased the extent of irreversible binding suggesting involvement of lysine residues for covalent binding.”

“In addition, it was demonstrated that, in the presence of ASA , the first binding site of BR on HSA was altered, but the parameters of binding did not become significantly modified, and thus the affinity of BR barely changed with and without ASA.”

“Furthermore, the results of synchronous fluorescence, resonance light scattering and circular dichroism of the binary and ternary systems showed that the binding of TMX and ASA to HSA could induce conformational changes in HSA.”

“The addition of capric acid or preincubation of HSA with acetylsalicylic acid or physostigmine impaired the catalysis to varying extents.”

“The fractional acetylsalicylic acid ASA ) hydrolysis at the completion of 20-minute incubations in suspensions of washed red blood cells (RBC) with human serum albumin (HSA) decreased sharply, from 0.6 at 0 gm/dl HSA to 0.2 at 5.0 gm/dl HSA.”

“More selective chemical modifications of HSA were realised with acetylsalicylic acid , diethylpyrocarbonate (DEP) and o-nitrophenylsulfenyl-chloride (NPS-C1).”

“The binding of ASA by HSA was markedly different from that of SA.”

“HUMAN serum albumin HSA ) is permanently altered after exposure to therapeutic concentrations of acetylsalicylic acid (ASA, aspirin) either in vitro or in vivo1–3.”