Possible Interaction: Aspartic Acid and Citrulline

“Under these physiological conditions, the condensation reaction of citrulline with aspartic acid may become ratelimiting in the operation of the urea cycle with the resultant accumulation of citrulline.”

“The data suggested that in livers of hydrazine-poisoned animals the condensation reaction of citrulline with aspartic acid to give argininosuccinic acid might become rate-limiting in the operation of the ornithine cycle and that the function of several other amino-acid metabolizing systems also might be disturbed.”

“The data were tentatively interpreted to indicate that under ordinary conditions the rate-limiting step in the ornithine cycle of liver may be the carbamylation of ornithine, but that in hydrazine-poisoned animals the condensation raction of citrulline with aspartic acid to give argininosuccinic acid might become rate-limitin.”

“Arginine methyl ester (99.5 and 97% inhibition), argininamide (99 and 96%), S-(2-aminoethyl) cysteine (… and 37%), aspartic acid β-methyl ester (38 and 40%), norvaline (0 and 3%), citrulline (9 and 7%), and asparagine (2 and … the incorporation of both arginine and aspartic acid , respectively, when … mixture.”

“Nature of Reaction Mechanism-Arginine is formed in the liver by an ATP-dependent’ condensation of citrulline with aspartic acid to form an intermediary condensation product, which is then split by a separate enzyme.”