Possible Interaction: Aspartate and Alpha-Ketoglutarate

“An increase of 1-aspartate concentration from 33 mM/1 to 200 mM/1 and of alpha-ketoglutarate from 6.7 mM/1 to 12 mM/1 elevated the enzymatic activity by 30-40%.”

“Glutamate and aspartate generally inhibit 2-oxoglutarate uptake, but under certain conditions may increase uptake.”

“In liver, 2-oxoglutarate is a potent inhibitor of oxalacetate transamination to aspartate and activation of this enzyme by calcium-mobilizing hormones leads to a stimulation of aspartate formation and gluconeogenesis.”

“Replacing Val37 with a less bulky alanine residue does not affect the maximal catalytic rate (kcat), but it does increase significantly the Michaelis constants for substrates in the overall transamination reaction between aspartate and 2-oxoglutarate.”

“Malate and other citric acid cycle dicarboxylates, but not aspartate and glutamate, may compete with 2-oxoglutarate for uptake into perivenous glutamine synthesizing hepatocytes.(ABSTRACT TRUNCATED AT 400 WORDS)”

“Previous studies from this laboratory showed that 2-oxoglutarate can inhibit aspartate production by isolated mitochondria.”

“L‐Glutamate and L‐as‐partate were comparatively strong inhibitors of γ‐keto‐glutarate and malate uptake.”

“30 μM. Oxidation of lactate is also sensitive to inhibitors of mitochondrial electron and energy transfer and it is enhanced by α-oxoglutarate plus aspartate.”

“The mitochondria readily oxidized aspartate (Asp) or alpha-KG individually with low rates, while they oxidized Asp and alpha-KG simultaneously with high rates, and this simultaneous oxidation was also inhibited by PLP.”

“A high degree of transamination was observed between 2 oxoglutarate and alanine, aspartate and ornithine.”