Possible Interaction: Asparagine and Oligosaccharides

“N-glycosylation, the enzymatic coupling of oligosaccharides to specific asparagine residues of nascent polypeptide chains, is one of the most widespread post-translational modifications.”

“It functions by modifying appropriate asparagine residues of proteins with oligosaccharide structures , thus influencing their properties and bioactivities.”

“The complexity of N-glycosylation is augmented by the fact that different asparagine residues within the same polypeptide may be modified with different oligosaccharide structures , and various proteins are distinguished from one another by the characteristics of their carbohydrate moieties.”

“In addition to the glycosylated glutamine, …-enriched antibody population showed that 4 asparagine residues : heavy chain Asn-162, Asn-360, and light chain Asn-164, both of which are present in … IgG2 constant domain sequences, and Asn-35, which was present in CDRL1, were also modified with oligosaccharide structures ….”

“Thus, the conformational space available to an N-linked oligosaccharide in a glycoprotein relative to the protein may depend to a large extent upon the flexibility of the asparagine side chain.”

“The biological addition of oligosaccharide moieties to asparagine residues of N-glycoproteins influences the properties and bioactivities of these macromolecules.”

“The biological addition of oligosaccharide structures to asparagine residues of N-glycoproteins influences the properties and bioactivities of these macromolecules.”