Possible Interaction: Asparagine and Histidine

“The data show that a single amino acid substitution of histidine with asparagine at position 99 in VH CDR3 region contributes to four times increase in binding affinity for the TAG72 antigen.”

“Through asparagine synthetase knockdown and altering of media asparagine concentrations, we show that intracellular asparagine levels regulate uptake of amino acids, especially serine, arginine and histidine.”

“In all these cases it seems that a histidine may catalyze the deamidation of the asparagine residues and that disturbing the folding of the EF corner will provide an extra flexibility favoring the reaction.”

“Under water stress asparagine , phenylalanine, serine, and histidine also declined compared to controls.”

“Substitution of this histidine by asparagine in Orf2* abolishes deacetylase activity.”

“ResultsCorrespondence analysis of the composition of all P. ingrahamii proteins showed that (1) there are 6 classes of proteins, … bulk proteins suggesting that, overall, they may have a lower hydrophobic character, and (3) there is strong opposition between asparagine … histidine and (4) one of the … are cold-specific proteins.”

“Replacement of histidine by asparagine residues strongly affected the kcat for beta-cyclodextrin-forming, coupling, and hydrolyzing activities, whereas it barely affected the Km values.”

“… asparagine … histidine so that the amide-NH2 group of asparagine occupies the same position as the pi-N of histidine, whereas the equivalent -NH2 group of … the pi-N of histidine that hydrogen bonds with ATP and that there is no significant electrostatic interaction between the histidine and ATP.”