Possible Interaction: Asparagine and Glutamine

“Two recent papers by Knott et al. (2018) and Pavlova et al. (2018) demonstrate that asparagine and glutamine work in concert to drive tumor growth and metastasis through modulation of cell survival, growth, and EMT regulatory pathways.”

“Replacing asparagines with glutamines created stronger suppressors.”

“Simultaneously replacement of all three asparagines with glutamines led to significantly reduced protein level as well as loss of transport activity.”

“The efficiency of reverse transcription was severely impaired by nonconservative substitution of Gln with Ala, while conservative substitution of Gln with Asn resulted in an approximately 70% loss of activity, a value similar to that observed with the Y183F mutation.”

“Substitution of asparagines for glutamines at amino acid positions 136 and 184 rendered these sites non-functional as shown by patch-clamp experiments.”

“ Glutamine uptake was markedly inhibited in the presence of L-alanine, L-asparagine , and L-serine.”

“A lack of glutaminase activity in these mutants also allowed examination of glutamine binding in studies of the ability of glutamine to inhibit the ammonia-dependent production of asparagine.”

“ L-glutamine also counteracted the effect of L-asparaginase partially, but to a lesser degree than L-asparagine.”

“The addition of glutamine reduced the net utilization of asparagine by both jejunal and ileal mixed bacteria.”

“ Asparagine and alanine produced electrophysiological and calcium changes that were at least as large as those caused by glutamine, but they were less effective GLP-1 secretagogues, suggesting that glutamine also potentiates secretion downstream of the calcium signal.”